CD33

Gene Symbol CD33
Entrez Gene 945
Alt Symbol SIGLEC-3, SIGLEC3, p67
Species Human
Gene Type protein-coding
Description CD33 molecule
Other Description CD33 antigen (gp67)|gp67|myeloid cell surface antigen CD33|sialic acid binding Ig-like lectin 3|sialic acid-binding Ig-like lectin 3
Swissprots F8WAL2 C9JEN7 Q8TD24 B4E3P8 P20138
Accessions AAK83654 CAD36509 CCF76941 CCF76942 CDM22283 EAW71994 EAW71995 EAW71996 P20138 AK090470 BAC03451 AK293934 BAG57312 AK304810 BAG65560 AK309558 AW007749 AY162464 AAO26206 BC028152 AAH28152 BP295158 BP303860 CA426969 CA748646 DQ891726 ABM82652 DQ894903 ABM85829 M23197 AAA51948 XM_011527531 XP_011525833 XM_011527532 XP_011525834 XR_935875 NM_001082618 NP_001076087 NM_001177608 NP_001171079 NM_001772 NP_001763
Function Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). {ECO:0000269|PubMed:10556798, ECO:0000269|PubMed:11320212}.
Subcellular Location Cell membrane; Single-pass type I membrane protein.
Tissue Specificity Monocytic/myeloid lineage cells.
Top Pathways Hematopoietic cell lineage

CD33 (E-6) - sc-376184 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD33 (E-6): sc-376184. Western blot analysis of CD33 expression in non-transfected: sc-117752 (A) and human CD33 transfected: sc-114798 (B) 293T whole cell lysates.

CD33 (6C5/2) - sc-53199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD33 (6C5/2): sc-53199. Western blot analysis of CD33 expression in AML-193 whole cell lysate under non-reducing conditions.

CD33 (B-9) - sc-374450 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD33 (B-9): sc-374450. Western blot analysis of CD33 expression in non-transfected: sc-117752 (A) and human CD33 transfected: sc-114798 (B) 293T whole cell lysates.

CD33 (CD33-4D3) - sc-59020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

CD33 (H-110) - sc-28811 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD33 (H-110): sc-28811. Western blot analysis of CD33 expression in human PBL whole cell lysate.

CD33 (P67.6) - sc-19660 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD33 (P67.6) PE: sc-19660 PE. FCM analysis of THP-1 cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

CD33 (M-90) - sc-28810 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD33 (M-90): sc-28810. Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.

CD33 (C-16) - sc-7038 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD33 (C-16): sc-7038. Western blot analysis of CD33 expression in non-transfected: sc-117752 (A) and human CD33 transfected: sc-114798 (B) 293T whole cell lysates.

CD33 (N-19) - sc-7039 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CD33 antibody - ab194662 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD33 antibody (ab194662) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : HepG2 cell lysateLane 3 : HCT116 cell lysate

Anti-CD33 antibody - ab203253 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue sections labeling CD33 with ab203253 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Anti-CD33 antibody - ab116717 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CD33 antibody (ab116717) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

Anti-CD33 antibody - ab123244 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CD33 antibody (ab123244) at 1 µg/ml + Human fetal heart lysate at 10 µg

Anti-CD33 antibody [3D6] - ab11031 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3D6

Anti-CD33 antibody [6C5/2] - ab11032 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 6C5/2
IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD33 antibody [6C5/2] (FITC) - ab187838 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 6C5/2

Anti-CD33 antibody [EPR4423] - ab134115 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4423
All lanes : Anti-CD33 antibody [EPR4423] (ab134115) at 1/1000 dilutionLane 1 : THP1 cell lysateLane 2 : U937 cell lysateLane 3 : HL60 cell lysateLane 4 : HepG2 cell lysateLane 5 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-CD33 antibody [HIM3-4] - ab2579 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone HIM3-4

Anti-CD33 antibody [HIM3-4] (Biotin) - ab21892 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone HIM3-4

Anti-CD33 antibody [HIM3-4] (CF405M) - ab119490 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone HIM3-4
ab119490, at 5 µl/106 cells, staining CD33 in normal Human peripheral blood cells by Flow Cytometry. Cells in the lymphocyte gate were used for the analysis.

Anti-CD33 antibody [HIM3-4] (PE/Cy5®) - ab169823 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone HIM3-4
Flow cytometric analysis of normal human peripheral blood cells labeling CD33 with ab169823 at 5&microl/105-106 cells (filled histogram) or PE-Cy5 Mouse IgG1 K Isotype Control (open histogram). Cells in the monocyte gate were used for analysis.

Anti-CD33 antibody [WM-53 (WM53)], prediluted (PerCP/Cy5.5®) - ab155352 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone WM-53 (WM53)
Staining of normal Human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or ab155352 (filled histogram). Cells in the monocyte gate were used for analysis.

Anti-CD33 antibody [WM-53(WM53)], prediluted (PE/Cy7 ®) - ab155348 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone WM-53(WM53)
Flow cytometric analysis of normal Human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE/Cy7 (blue histogram) or ab155348 (purple histogram). Cells in the monocyte gate were used for analysis.

Anti-CD33 antibody [WM53] - ab119860 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone WM53

Anti-CD33 antibody [WM53] - ab30371 from Abcam

Prices $381.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone WM53
ab30371 staining human peripheral blood monocytes by flow cytometry
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