ESPL1

Gene Symbol ESPL1
Entrez Gene 9700
Alt Symbol ESP1, SEPA
Species Human
Gene Type protein-coding
Description extra spindle pole bodies homolog 1 (S. cerevisiae)
Other Description caspase-like protein ESPL1|extra spindle poles like 1|extra spindle poles-like 1 protein|separin|separin, cysteine protease
Swissprots Q14674
Accessions EAW96681 EAW96682 Q14674 AI458447 AK128350 AK299423 BAG61403 AK316135 BAH14506 AW009863 AY455930 AAR18247 BC047603 BC172238 AAI72238 BC172570 AAI72570 BG328178 BP244662 D79987 BAA11482 XM_006719705 XP_006719768 XM_011539024 XP_011537326 XM_011539025 XP_011537327 XR_944842 NM_012291 NP_036423
Function Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. {ECO:0000269|PubMed:10411507, ECO:0000269|PubMed:11509732}.
Subcellular Location Cytoplasm. Nucleus.
Top Pathways Cell cycle, Oocyte meiosis

Separase (H-300) - sc-25839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Separase (H-300): sc-25839. Western blot analysis of Separase expression in MDA-MB-231 whole cell lysates (A) and HeLa nuclear extract (B).

Separase (B-4) - sc-390314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Separase (B-4): sc-390314. Western blot analysis of Separase expression in HeLa nuclear extract (A) and HEL 92.1.7 whole cell lysate (B).

Separase (C-20) - sc-27219 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Separase (N-15) - sc-27217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Separase (N-15): sc-27217. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p-Separase (Ser 1073) - sc-130217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Separase (Ser 1073): sc-130217. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.

Anti-Separase (phospho S1176) antibody - ab59991 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab59991 at 1/50-1/100 dilution staining Separase in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-Separase (phospho S1501) antibody - ab60027 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab60027 at 1/50 dilution staining formalin fixed, paraffin embedded human hepatocarcinoma tissue. Peroxidase conjugated secondary antibody followed by AEC staining.

Anti-Separase (phospho S748) antibody - ab59992 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab59992 at 1/50-1/100 dilution staining Separase in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Anti-Separase (phospho S801) antibody - ab52210 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Paraffin-embedded human colon carcinoma tissue stained for Separase using ab52210 (1/100). The right hand panel represents a negative control where ab52210 was pre-incubated with the immunising (blocking) peptide.

Anti-Separase antibody - ab3762 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Metaphase HeLa cells were labelled with anti-ACA (anti-centromeric antigen) and anti-Separase (ab3762). ab3762 was used at a working concentratin of 1µg/ml. The arrows indicate the position of the centrosomes and demonstrate a spatial relationship between Separase and the centrosomes during metaphase. In the final panel DAPI is pseudo-coloured blue, while ACA and Separase are green and red respectively. 100x magnification.

Anti-Separase antibody - ab199153 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Separase with ab199153 at 1/40 dilution.

Anti-Separase antibody - ab52158 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Separase antibody (ab52158) at 1/500 dilutionLane 1 : extracts from A431 cellsLane 2 : extracts from A431 cells, with immunizing peptide

Anti-Separase antibody - ab85685 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Separase antibody (ab85685) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-Separase antibody [2329C1a] - ab84868 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2329C1a
Anti-Separase antibody [2329C1a] (ab84868) + Immunogen (Recombinant Human Separase fragment)

Anti-Separase antibody [XJ11-1B12] - ab16170 from Abcam

Prices $398.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone XJ11-1B12
ab16170 stained MCF7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16170 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-ESPL1 / Separase Antibody (Ser1126, AP) - LS-C282621 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (Ser1126, Biotin) - LS-C282623 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (Ser1126, APC) - LS-C282622 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (Ser1126, Azide-free, HRP) - LS-C282624 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (Ser1126, FITC) - LS-C282626 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (Ser1126, PE) - LS-C282627 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (aa1100-1150) - LS-C288966 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (Ser1176, Biotin) - LS-C284695 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (aa1425-1475) - LS-C288970 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ESPL1 / Separase Antibody (phospho-Ser748) - LS-C328625 from LifeSpan Bioscience

Prices $425.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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